Summary
Type: SPE
Application #: 304150
Catalog#: 730004 - SPE Cartridges, Chromabond, C18, 6ml, PP, 500mg, 30/pk
Detection: HPLC with RP conditions:
Stationary phase: CC 250/4 Nucleosil 100-5 C18 (Cat. No: 721662.40), guard column CC 8/4 Nucleosil 100-5 C18 (Cat. No. 721602.40).
Mobile phase: Eluent A: 0.085% phosp
Conditions: Column conditioning: 2 ml methanol, then 2 ml 7 mM EDTA solution.
(Do not let run the column dry.)
Sample aspiration: 100 ml sample solution is passed through the column by vacuum or pressure (3 ml/min)
Drying 1: 60 min, air stream
Elution 1: 4 ml methanol (1ml/min)
Drying 2: 30 min, air stream
Washing: 4 ml acidic water (pH 2, adjusted with HCl, 3 ml/min)
Drying 3: 60 min, air stream
Elution 2: 4 ml methanol (1ml/min)
Evaporation to dryness
Solution of eluates in 1 ml water
Authors: Kümmerer K et al
Source: Antibiotikaresistenz - Vorkommen und Uuml;bertragung in Abwasser Oberflächenwasser und Trinkwasser Teil 2 Abschlussbericht Freiburg (Germany) Dezember 2002